Culex Sampling Options
Blood Sampling in Rats
A user-defined method allows collection of blood samples for PK and bioavailability studies. Sample sizes are dependent on the choice of collector below. Choose a method for Whole Blood or No Blood Waste with sample times starting a 0 minutes. Use the program to simultaneously collect behavior. Utilize the Empis for automated dosing capabilities.
The Small Volume Fraction Collector (MD-1201)
- Collects samples from 5µL to 250µL
- Required for sampling in mice, but is also ideal for rats.
- Can be used interchangeably to sample blood or dialysates
- Optional refrigeration keeps samples at 4°C
- Dual cannula sampling capabilities when used for microdialysis
- Holds up to 48 samples at one time.
The Large Volume Fraction Collector (MD-1202)
- Collects samples from 50µL to 1.5mL
- Ideal for larger rodents including diabetic rats and guinea pigs
- Can be used interchangeably to sample blood or perform automated bile sampling.
- Optional refrigeration keeps samples at 4°C
- Holds up to 26 samples at one time
- Dried blood spot collection option
Blood Sampling in Mice
Sample from the carotid artery or jugular vein. To minimize blood loss, mice are sampled with a No Blood Waste method. Samples can be taken as close as 3 minutes apart with volumes as small as 5µL. These small volumes permit bioavailability studies in individual mice. Run your method anytime of the day or night and use the program to simultaneously collect behavior.
Blood Sampling in Large Animals
The new Culex-L expands the capabilities of the Culex to permit automated sampling in large animals. The system was developed with swine, but can be applied to many other species. The innovative movement responsive caging facilitates collection of multiple streams of data to get the most out of your research. Collect large volume blood samples anytime, with less stress for both the subject and the technician.
Blood Sampling in Large Animals
Microdialysis and Ultrafiltration
Use the Raturn platform to create continuous connections from pump to probe to collector for microdialysis and ultrafiltration experiments. Avoid tangled lines, leaky swivels, and time consuming cleanup. Pair the dialysate/ultrafiltrate collection with blood collection on the Culex to achieve a complete picture of both systemic and target concentrations. For microdialysis experiments, add in the Empis automated dosing system to automatically refill and perfuse your probe and to collect behavior simultaneously. Our automated, refrigerated fraction collector keeps your samples at 4°C until you are ready to collect them.
Microdialysis and Ultrafiltration
Automated Bile Collection
Collect bile at programmed intervals rather than a single continuous drain. The Empis system directs flow, but does not actively collect from the animal. During user-defined collection periods, replacement bile salts are directed toward the subject. While not collecting, the bile is naturally re-circulated via the duodenal catheter. Discrete bile samples are collected into vials in a refrigerated collector. The entire system is a closed loop, making cleanup a breeze. Use the Culex to perform simultaneous blood sampling and use a metabolic cage to collect urine and feces for a full metabolic profile
Metabolite Collection
Easily collect both urine and feces in rats and mice. Use metabolic cages on a Metabolism Stand, a Stand Alone Raturn, or with any of the Culex cart configurations. Easily separate urine and feces, storing urine in the i-cup chilling chamber. Use this caging to collect metabolites during any Culex study.
i-cup Chilling Chamber
The i-cup (CX-1400) is a portable, non-electric vial chiller used to refrigerate urine as it is collected and maintain it below 4°C throughout the day or overnight. It was developed for metabolic collections with the Culex automated blood sampling system but can be used anywhere liquids need to be kept cold for extended periods.
Simply place the i-cup in a -80°C freezer for 6 hours or -20°C freezer for 30 hours. Remove the i-cup from the freezer and it will keep your liquids cold for a minimum of 16 hours.
The new larger cold well now accommodates 50 mL tri-pour beakers, 42 mL capped urine collection vials (CX-1211), as well as 20 mL scintillation vials (PL-4000).
| Chill temperature | 0 – 3°C |
| Chill duration | 16 hours minimum |
| Vial capacity | 1 |
| Vial size | up to 50 mL |
| Outer dimensions | 7.2 inch diameter x 2.8 inch height (183 mm diameter x 71 mm height) |
| Cold well dimensions | 1.57 inch diameter x 2.2 inch height (40 mm diameter x 56 mm height) |
| Material | White ABS |
| Freeze at | -80°C for 6 hours or -20°C for 30 hours |